VIII-6G10細胞

細胞形態(tài)： **樣

**類型： IgG1; kappa light chain

ATCC Number： HB-10519?

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

運輸方式： 凍存運輸

Designations: VIII-6G10

Depositors: Fred Hutchinson Cancer Res. Cntr.

VIII-6G10細胞Isotype: IgG1; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against vascular cell adhesion molecule 1 (VCAM-1, VCAM 1) of human and macaque

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VIII-6G10細胞Tumorigenic: Yes

Comments: Animals were immunized with IL-4 and IL-1 beta activated endothelial cells isolated from mesenteric lymph nodes of Macaca nemestrina.

Spleen cells were fused with NS-1 myeloma cells.

The antibody is specific for VCAM-1 and blocks up to 90% of lymphocyte adhesion to microvascular endothelial cells.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 25 mM HEPES, 2 mM L-glutamine and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: VIII-6G10細胞Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22026: Masinovsky B, et al. IL-4 and TNF induce mAb 6G10-recognized expression on bone marrow stromal cells. US Patent 5,206,345 dated Apr 27 1993

23475: Masinovsky B, et al. IL-4 acts synergistically with IL-1 beta to promote lymphocyte adhesion to microvascular endothelium by induction of vascular cell adhesion molecule-1. J. Immunol. 145: 2886-2895, 1990. VIII-6G10細胞PubMed: 1698865