McCoy [McCoy B]細(xì)胞

組織來源： unknown

細(xì)胞類型： 成纖維細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

ATCC Number： CRL-1696?

細(xì)胞形態(tài)： 成纖維樣

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

Designations: McCoy [McCoy B]

Depositors: CDC

McCoy [McCoy B]細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Tissue: unknown

Cell Type: fibroblast

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Comments: Little descriptive information about the origin of the McCoy cells appears in the literature. They were first mentioned by Pomerat, et al. [26143]. McCoy [McCoy B]細(xì)胞The cells were reported to have originated from the synovial fluid in the knee joint of a patient suffering from degenerative arthritis. In ca. 1965, Defendi, et al., showed that McCoy cells (designated McCoy A) were indeed human cells. However, another subline (designated McCoy B) was, in fact, of mouse origin and possessed marker chromosomes characteristic of strain L mouse fibroblasts. McCoy cells presumed to be human, but which actually are mouse cells, have been disseminated from laboratory to laboratory throughout the world. Initial interest in McCoy cells followed the demonstration by Gordon and Quan [PubMed ID: 14268619] and Gordon, et al., [PubMed ID: 4110420], that ionizing radiation (cobalt-60) greatly increased the susceptibility of McCoy cells to infection by chlamydia strains. A culture of the so-called McCoy cell line was received from the Centers for Disease Control, Cell Culture Department, Atlanta, GA in March, 1984. Documentation as to origin or passage history was not available. The cells have been used to propagate laboratory strains of the 15 recognized serotypes of Chlamydia trachomatis. The cell line has been satisfactory for chlamydia growth for at least 43 passages at ATCC .

The cells are susceptible to chlamydia strains, and can be used to propagate chlamydia.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: McCoy [McCoy B]細(xì)胞Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell supension to new culture vessels.

Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 2 to 3 times per week

Related Products: McCoy [McCoy B]細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

Cell culture tested DMSO:ATCC 4-X

References: 26143: Pomerat CM, et al. Irradiation of cells in tissue culture. I. Giant cell induction in strain cultures versus elements from primary explants. Z. Zellforsch. 47: 158-174, 1957.

26144: Gordon FB, Quan AL. Isolation of the trachoma agent in cell culture. Proc. Soc. Exp. Biol. Med. 118: 354-359, 1965. PubMed: 14268619

26145: Gordon FB, et al. Effect of ionizing irradiation on susceptibility of McCoy cell cultures to Chlamydia trachomatis. Appl. Microbiol. 23: 123-129, 1972. PubMed: 4110420

33114: Yasin B, et al. Susceptibility of Chlamydia trachomatis to protegrins and defensins. Infect. Immun. 64: 709-713, 1996. PubMed: 8641770