MDA-kb2細(xì)胞

細(xì)胞形態(tài)： 上皮樣

細(xì)胞類型： 上皮細(xì)胞

器官來源： **

是否是腫瘤細(xì)胞： 0

物種來源： 人

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

運(yùn)輸方式： 凍存運(yùn)輸

年限： 48 years *****

ATCC Number： CRL-2713?

Designations: MDA-kb2

Depositors: VS Wilson

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: MDA-kb2細(xì)胞adherent

Organism: Homo sapiens deposited as human

Morphology: epithelial

Source: Organ: mammary gland; breast

Cell Type: epithelial

Cellular Products: luciferase ( [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors (GR) and androgen receptors (AR).)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: November 25, 1998

Applications: MDA-kb2細(xì)胞assay of androgen receptor ( [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.)

Receptors: androgen receptor [57526]

glucocorticoid receptor, positive [57526]

DNA Profile (STR): Amelogenin: X

CSF1PO: 10,12

D13S317: 12

D16S539: 9

MDA-kb2細(xì)胞D5S818: 11

D7S820: 10

THO1: 6

TPOX: 10

vWA: 17,18

Age: 48 years *****

Gender: female

Ethnicity: Caucasian

Comments: Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.

Propagation: MDA-kb2細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 100%

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020

parental cell line:ATCC HTB-131

References: 57526: Wilson VS, et al. MDA-kb2細(xì)胞A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974