4B2細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

ATCC Number： HB-196?

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG2a

Designations: 4B2

Depositors: RM Steinman

Isotype: IgG2a

Biosafety Level: 1

4B2細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: 4B2細(xì)胞immunoglobulin; monoclonal antibody; against the human T200 common leukocyte antigen (CD45)

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Tumorigenic: Yes

Comments: Animals were immunized with human T cells.

Spleen cells were fused with P3U1 myeloma cells.

The antibody reacts with all types of human leukocytes, but not with non-leukocytes.

The antibody binds to protein A.

Tested and found negative for ectromelia virus (mousepox).

Propagation: 4B2細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 21881: . Leucocyte typing III: white cell differentiation antigens. Oxford: Oxford Univ.4B2細(xì)胞 Press; 1987.