DEC-205 [NLDC-145]細胞
是否是腫瘤細胞： 0
物種來源： 大鼠
ATCC Number： HB-290?
細胞形態(tài)： **樣
**類型： IgG2a
運輸方式： 凍存運輸
數(shù)量： 大量
生長狀態(tài)： 懸浮生長
Designations: DEC-205 [NLDC-145]
Depositors: RM Steinman, G Kraal
Isotype: IgG2a
DEC-205 [NLDC-145]細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: immunoglobulin; monoclonal antibody; against the mouse DEC-205 antigen (mouse dendritic cell antigen)
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Comments: DEC-205 [NLDC-145]細胞Animals were immunized with mouse lymph node stroma.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody reacts with an antigen that is expressed at high levels on mouse dendritic cells and thymic cortical epithelial cells.
The antigen is a 205,000 dalton group VI C type molecule that mediates adsorptive endocytosis and antigen presentation.
The antibody reacts with and stains mouse dendritic cells and epidermal Langerhans cells.
The cells are capable of being adapted to serum free growth in using Nutridoma medium.
Propagation: DEC-205 [NLDC-145]細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
References: 22819: Kraal G, et al. Langerhans' cells, veiled cells, and interdigitating cells in the mouse recognized by a monoclonal antibody. J. Exp. Med. 163: 981-997, 1986. DEC-205 [NLDC-145]細胞PubMed: 3950549
