RFL-6細(xì)胞

器官來源： 肺

ATCC Number： CCL-192?

相關(guān)**： 正常

品系： Sprague-Dawley

年限： 18 days gestation

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 成纖維樣

RFL-6細(xì)胞細(xì)胞類型： 成纖維細(xì)胞

生長狀態(tài)： 貼壁生長

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

數(shù)量： 大量

Designations: RFL-6

Depositors: ML Macy

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

RFL-6細(xì)胞Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: fibroblast

Source: Organ: lung

Strain: Sprague-Dawley

Disease: normal

Cell Type: RFL-6細(xì)胞fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 18 days gestation

Propagation: ATCC complete growth medium: Ham's F12K medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: RFL-6細(xì)胞Twice per week

Remove medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit for 10 to 12 minutes at 37C. Add fresh medium, aspirate and dispense into new flasks. Subculture every 5 to 6 days.

Preservation: Freeze medium: complete growth medium, 95%; DMSO, 5%