FW11-9-2細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG2a

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： HB-256?

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

Designations: FW11-9-2

Depositors: CR Mackay

Isotype: IgG2a

FW11-9-2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

FW11-9-2細(xì)胞Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against the v6 variant of human CD44

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: FW11-9-2細(xì)胞Animals were immunized with a fusion protein corresponding to the variant region of human CD44 (exons 3v to 10v).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody is specific for the product of the 6v exon.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22355: Mackay CR, et al. FW11-9-2細(xì)胞Expression and modulation of CD44 variant isoforms in humans. J. Cell Biol. 124: 71-82, 1994. PubMed: 7507492