WIL2-S細(xì)胞

細(xì)胞形態(tài)： **樣

年限： 5 years

ATCC Number： CRL-8885?

相關(guān)**： 其他**

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

器官來(lái)源： 脾

數(shù)量： 大量

細(xì)胞類型： 其他細(xì)胞類型

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

WIL2-S細(xì)胞Designations: WIL2-S

Depositors: Lovelace Medical Foundation

Biosafety Level: 2

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Organ: spleen

Disease: hereditary spherocytosis

Cell Type: lymphoblast B lymphoblast;

Permits/Forms: WIL2-S細(xì)胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11

D16S539: 11,12

D5S818: 12,13

D7S820: 9,11

WIL2-S細(xì)胞F13A01: 6,7

F13B: 10

FESFPS: 11,12

LPL: 9,10

THO1: 8,9.3

TPOX: 8,11

vWA: 17,20

Age: 5 years

Gender: male

Ethnicity: WIL2-S細(xì)胞Caucasian

Comments: This is a HAT sensitive variant of the WIL2 human B cell line (see also ATCC CRL-8155).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: WIL2-NS, ATCC CRL-8155

TK6, ATCC CRL-8015

LTR228 , ATCC HB-8502