DATK32細(xì)胞

ATCC Number： HB-294?

是否是腫瘤細(xì)胞： 0

物種來源： 大鼠

細(xì)胞形態(tài)： **樣

**類型： IgG2a

數(shù)量： 大量

運輸方式： 凍存運輸

生長狀態(tài)： 懸浮生長

Designations: DATK32

Depositors: EC Butcher, DP Andrew

Isotype: IgG2a

DATK32細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus (B cell); Mus musculus (myeloma) deposited as rat (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against murine alpha 4, beta 7 integrin

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Comments: Animals were immunized with cells of the TK1 murine T cell lymphoma.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with the mouse lymphocyte mucosal homing receptor.

It is specific for mouse alpha 4, beta 7 heterodimers, and can induce alpha 4, beta 7 dependent lymphocyte aggregation.

The antibody inhibits alpha 4, beta 7 mediated adhesion activities including binding to MAdCAM-1, and alpha 4, beta 7 dependent VCAM-1 and fibronectin binding.

DATK32 is useful in flow cytometry methods using sensitive second stage reagents, but is ineffective in immunohistological staining.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 4.5 g/L glucose,15 mM HEPES, 2 mM L-glutamine, 0.01 mM nonessential amino acids and 0.05 mM 2-mercaptoethanol, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. DATK32細(xì)胞Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22636: Berlin C, et al. Alpha 4 beta 7 integrin mediates lymphocyte binding to the mucosal vascular addressin MAdCAM-1. Cell 74: 185-195, 1993. PubMed: 7687523

23486: DATK32細(xì)胞Andrew DP, et al. Distinct but overlapping epitopes are involved in alpha 4 beta 7- mediated adhesion to vascular cell adhesion molecule-1, mucosal addressin-1, fibronectin, and lymphocyte aggregation. J. Immunol. 153: 3847-3861, 1994. PubMed: 7523506