Reh細(xì)胞

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

是否是腫瘤細(xì)胞： 0

物種來源： 人

ATCC Number： CRL-8286?

相關(guān)**： 其他**

運(yùn)輸方式： 凍存運(yùn)輸

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

Designations: Reh

Depositors: Univ. Pennsylvania

Biosafety Level: 1

Reh細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Source: Disease: acute lymphocytic leukemia (non-T; non-B)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Antigen Expression: Reh細(xì)胞CD3 A (17%) B (17%) C (20%), CD4 (15%), CD10 (55%)

DNA Profile (STR): D5S818: 11, 13

D13S317: 11, 13

D7S820: 9, 12

D16S539: 9

vWA: 14, 15

THO1: 7

TPOX: 8

CSF1PO: 10, 11, 12

Amelogenin: X

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Reh細(xì)胞Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10(5) cells/ml and maintain between 1 X 10(5) and 1 X 10(6) cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: 95% (v/v) FBS; 5% (v/v) DMSO

Storage temperature: Liquid nitrogen vapor phase

References: 22700: Greaves M, Janossy G. Patterns of gene expression and the cellular origins of human leukaemias. Biochim. Biophys. Acta 516: 193-230, 1978. Reh細(xì)胞PubMed: 365240