HGF-1細胞

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 人

年限： 28 years

ATCC Number： CRL-2014?

相關(guān)**： 正常

組織來源： gingiva

細胞形態(tài)： 成纖維樣

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

Designations: HGF-1

Depositors: BS McAllister

Biosafety Level: 1

Shipped: frozen

HGF-1細胞Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Source: Tissue: gingiva

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: October, 1989

Applications: HGF-1細胞Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.

Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells.

Receptors: bradykinin, expressed

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11

D13S317: 8,12

D16S539: 11,13

D5S818: 12

D7S820: 10

THO1: 6,7

TPOX: 8,11

vWA: 17,18

Age: 28 years

Gender: male

Ethnicity: Caucasian

HGF-1細胞Comments: Bradykin receptors are present on the cells at an apparent density of 22000 per cells and dissociation constant (Kd) of 1.2 nM. The receptors are coupled to a signaling pathway involving phospholipase C and A2 and calcium channel effectors.

Epidermal growth factor (EGF) and platelet derived growth factor (PDGF) stimulate DNA synthesis and proliferation in HGF-1 cells. Bradykinin treatment blocks the proliferation stimulation by EGF and PDGF.

The cells were deposited to ATCC at an unknown passage level. A longevity study performed by ATCC showed that the depositor?s material was capable of growing for an additional 22 population doublings (i.e., unknown + 22) before the onset of senescence.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: HGF-1細胞liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

recommended serum:ATCC 30-2020

References: 23126: McAllister BS, et al. Bradykinin receptors and signal transduction pathways in human fibroblasts: integral role for extracellular calcium. Arch. Biochem. Biophys. 304: 294-301, 1993. PubMed: 7686736

23527: McAllister BS, et al. Bradykinin inhibition of EGF- and PDGF-induced DNA synthesis in human fibroblasts. Am. J. Physiol. 265: C477-C484, 1993. PubMed: 8396328