BNL CL.2細(xì)胞

器官來源： 肝

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

數(shù)量： 大量

生長狀態(tài)： 貼壁生長

品系： BALB/c

年限： embryo

ATCC Number： TIB-73?

相關(guān)**： 正常

Designations: BNL CL.2

Depositors: P Patek, J Collins, M Cohn

BNL CL.2細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology:

Source: Organ: liver

Strain: BALB/c

Disease: normal

BNL CL.2細(xì)胞Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: No

Age: embryo

Comments: The line was selected to grow in medium containing ornithine and phenylalanine in place of arginine and tyrosine.

Tested and found negative for ectromelia virus (mousepox).

Propagation: BNL CL.2細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, add fresh 0.25% trypsin for 1 minute, remove trypsin and let the culture sit at room temperature until the cells detach (5 to 10 minutes). Add fresh medium, aspirate and dispense into new flasks.

Related Products: derivative:ATCC TIB-74

References: 22905: Patek PQ, et al. BNL CL.2細(xì)胞Transformed cell lines susceptible or resistant to in vivo surveillance against tumorigenesis. Nature 276: 510-511, 1978. PubMed: 723934