alphaTC1 Clone 9細(xì)胞

運輸方式： 凍存運輸

ATCC Number： CRL-2350?

相關(guān)**： 其他**

器官來源： 胰腺

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

品系： (C57BL/6J x DBA/2)F2

細(xì)胞形態(tài)： 上皮樣

Designations: alphaTC1 Clone 9

alphaTC1 Clone 9細(xì)胞Depositors: EH Leiter

Biosafety Level: 2 [Cells Contain PAPOVAVIRUS (SV-40) ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Mus musculus

Morphology: epithelial

Source: Strain: (C57BL/6J x DBA/2)F2

Organ: pancreas

Disease: adenoma

Cell Type: alpha cell;

Cellular Products: glucagon

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: alphaTC1 Clone 9細(xì)胞These cells are distributed for research purposes only. 1. The alphaTC1 Clone 9 cell line was deposited for research purposes only. Neither the cell line nor products derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or other warranty, express or implied. 2. Any proposed commercial use of these cells or products produced by them must first be negotiated with Edward H. Leiter, Telephone: 207-288-6370, FAX: 207-288-6077. 3. In all papers reporting any use of these cells or derived products, a direct reference will be made to the publications below.

Antigen Expression: H-2b

H-2d

Comments: alphaTC1 clone 9 is a pancreatic alpha cell line cloned from the alpha TC1 cell line which was derived from an adenoma created in transgenic mice expressing the SV40 large T antigen oncogene under the control of the rat preproglucagon promoter. [38839]

Though the parental alphaTC1 cell line produces glucagon and considerable quantities of insulin and preproinsulin mRNA, the clonal line is more differentiated and produces glucagon but not insulin or preproinsulin mRNA. [38840]

Rat recombinant gamma-interferon or mouse recombinantinterleukin-1 individually inhibited glucagon synthesis in alphaTC1 clone 9 cells but did not cause inhibition of DNA synthesis.

The combination of these cytokines caused marked inhibition of DNA and glucagon synthesis in alphaTC1 clone 9 cells. [38840]

This cell line is heterozygous for MHC alleles derived from both parental strains used in construction of the transgenic mice [C57BL/6J(H-2b] and DBA/2J(H-2d)]. [38840]

This cell line is useful for studying glucagon biosynthesis and alpha cell sensitivity to cytokines.

Propagation: ATCC complete growth medium: The base medium for this cell line is Dulbecco's Modified Eagle's Medium (Invitrogen Cat. No. 31600-034). alphaTC1 Clone 9細(xì)胞To make the complete growth medium, add the following components to the base medium:

heat-inactivated fetal bovine serum (FBS) to a final concentration of 10%

15 mM HEPES

0.1 mM non-essential amino acids

0.02% bovine serum albumin

1.5 g/L sodium bicarbonate

3.0 g/L glucose

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol:Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

NOTE: warm all solutions to 37.0°C prior to use

Transfer all medium and floating cells from flask to a 50 ml centrifuge tube.

Adherent cells are removed using Cell Dissociation Buffer (an enzyme free buffer; Invitrogen, Catalog No. 13150-016). Add 5.0 ml of diluted cell dissociation buffer per 75 sq cm flask and gently rock flask to bathe the cells at room temperature for 1 to 2 minutes.

Allow the flask to remain at room temperature for 1 to 5 additional minutes until cells have detached from the flask.

Firmly tap the flask against palm of hand to dislodge cells.

Add 10.0 ml of fresh medium per 75 cm2 flask and triturate up and down directing the stream along the growth surface of the flask to dislodge the cells and break up some of the clumps.

Transfer these cells to the centrifuge tube from Step 1. Centrifuge at 125 xg for 5 to 10 minutes. Remove medium and resuspend pellet in fresh complete medium.

Add appropriate aliquots of cell suspension to new culture vessels.

Incubate cultures at 37.0°C.

Subcultivation Ratio:alphaTC1 Clone 9細(xì)胞 A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Related cell line: ATCC CRL-2934 (alpha

References: 38839: Powers AC, et al. Proglucagon processing similar to normal islets in pancreatic alpha-like cell line derived from transgenic mouse tumor. Diabetes 39: 406-414, 1990. PubMed: 2156740

38840: Hamaguchi K, Leiter EH. Comparison of cytokine effects on mouse pancreatic alpha-cell and beta-cell lines. Viability, secretory function, and MHC antigen expression. Diabetes 39: 415-425, 1990. PubMed: 2108069