ACT IV細(xì)胞
ATCC Number： HB-81?
細(xì)胞形態(tài)： **樣
**類(lèi)型： IgG1
運(yùn)輸方式： 凍存運(yùn)輸
數(shù)量： 大量
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 小鼠
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
Designations: ACT IV
Depositors: P Parham
Isotype: IgG1
ACT IV細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)
Morphology: lymphoblast
Source: Cell Type: hybridoma: B lymphocyte;
Cellular Products: ACT IV細(xì)胞immunoglobulin; monoclonal antibody; against actin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Comments: Animals were immunized with actin from Dictyostelium discoideum.
Spleen cells were fused with NS-1 myeloma cells.
The antibody reacts with actin from prokaryotic organisms at an epitope distinct from that recognized by ACT I (ATCC HB-80).
The antibody does not cross-react with eukaryotic actins.
ACT IV細(xì)胞Tested and found negative for ectromelia virus (mousepox).
Propagation: ATCC complete growth medium: RPMI 1640 medium with 0.1 mM hypoxanthine, 400 nM aminopterin and 0.016 mM thymidine, 80%; fetal bovine serum, 20%
Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.
Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2001
Recommended serum: ATCC 30-2020
HAT (Hypoxanthine-Aminopterin-Thymidine): ATCC 69-X
References: 986: Simpson PA, et al. ACT IV細(xì)胞Monoclonal antibodies prepared against Dictyostelium actin: characterization and interactions with actin. J. Cell Biol. 99: 287-295, 1984. PubMed: 6203918
