GPC-16細(xì)胞
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
細(xì)胞形態(tài)： 上皮樣
器官來(lái)源： 結(jié)腸
ATCC Number： CCL-242?
相關(guān)**： 大腸癌
數(shù)量： 大量
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 豚鼠
Designations: GPC-16
Depositors: RW O'Donnell
GPC-16細(xì)胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cavia porcellus
Morphology: epithelial
Source: Organ: colon
Disease: colorectal adenocarcinoma
Cellular Products: keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. GPC-16細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
Tumorigenic: Yes
Comments: PAS, positive.
The cells are positive for keratin by immunoperoxidase staining.
Propagation: GPC-16細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: 1 to 2 times per week
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 23041: O'Donnell RW, Cockerell GL. GPC-16細(xì)胞Establishment and biological properties of a guinea pig colonic adenocarcinoma cell line induced by N-methyl-N-nitrosourea. Cancer Res. 41: 2372-2377, 1981. PubMed: 7237435
