N418細(xì)胞

細(xì)胞形態(tài)： **樣

**類型： IgG

數(shù)量： 大量

運輸方式： 凍存運輸

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

生長狀態(tài)： 懸浮生長

ATCC Number： HB-224?

Designations: N418

Depositors: RM Steinman

Isotype: IgG

Biosafety Level: 1

N418細(xì)胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; N418細(xì)胞against mouse leukocyte integrin (CD11c)

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Comments: Animals were immunized with mouse dendritic cells.

Spleen cells were fused with Sp2/0 myeloma cells.

The antibody reacts precipitates a 150000/90000 dalton CD11/CD18 heterodimer (see also ATCC HB-226) from murine dendritic cells.

The N418 epitope may be the mouse equivalent to human CD11c.

The N418 epitope is detected only on dendritic cells and is not present on peritoneal macrophages, anti-immunoglobulin induced B lymphoblasts or bulk lymph node cells.

Propagation: N418細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Preservation: Freeze medium: culture medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

References: 1793: Metlay JP, et al. N418細(xì)胞The distinct leukocyte integrins of mouse spleen dendritic cells as identified with new hamster monoclonal antibodies. J. Exp. Med. 171: 1753-1771, 1990. PubMed: 2185332