MDBK (NBL-1)細胞

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

細胞形態(tài)： 上皮樣

ATCC Number： CCL-22?

相關(guān)**： 正常

年限： *****

是否是腫瘤細胞： 0

物種來源： 牛

運輸方式： 凍存運輸

器官來源： 腎臟

Designations: MDBK (NBL-1)

MDBK (NBL-1)細胞Depositors: S Madin, NB Darby

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Bos taurus

Morphology: epithelial

Source: Organ: kidney

Disease: normal

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: MDBK (NBL-1)細胞detection and quantitation of replication competent retroviruses [92434]

transfection host

Virus Resistance: poliovirus 2

Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 60. The stemline chromosome number is hypodiploid with 2S component occurring at 5%., There were a total of 11-14 marker chromosomes (4-5 metacentric, 3-4 submetacentric and 4-5 acro-telocentric) common to most hypodiploid metaphases. The X was monosomic. Neither HSR chromosomes nor DM's were seen.

Age: *****

Gender: male

Comments: The MDBK cell line was derived from a kidney of an apparently normal ***** steer, February 18, 1957, by S.H. Madin and N.B. Darby.

It was ascertained in 1973 that the MDBK line was infected with bovine viral diarrhea virus (BVDV).

In 1982 R.A. Van Deusen of the NVSL, USDA, Ames, Iowa informed the ATCC that they had found an MDBK free of BVDV infection.

The cells were originally obtained in July, 1967 at passage 96 from the ATCC as a frozen ampule (F-145). Extensive testing (35 passages) by the NVSL found no evidence of any viral contamination in this particular progeny of MDBK.

The cell line was submitted to the American Type Culture Collection by R.A. Van Deusen in passage 110 in August, 1982.

The cells are positive for keratin by immunofluorescence.

MDBK (NBL-1)細胞The line has been shown to be free of bovine viral diarrhea virus (BVDV). [22883]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: horse serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Twice per week

Rinse the cell sheet twice with fresh 0.25% trypsin, 0.03% EDTA solution, and remove the trypsin solution. Allow the flask to stand at room temperature for 10 to 15 minutes, add fresh medium, aspirate and dispense into new flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

References: 22217: Madin SH, Darby NB Jr.. Established kidney cell lines of normal ***** bovine and ovine origin. Proc. Soc. Exp. Biol. Med. 98: 574-576, 1958. PubMed: 13567776

22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438

28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321

32728: Russell DW, Miller AD. MDBK (NBL-1)細胞Foamy virus vectors. J. Virol. 70: 217-222, 1996. PubMed: 8523528

92434: USEPA Manual of Methods for Virology - EPA publication. Washington, DC:Environmental Protection Agency;EPA EPA 600/4-84/013 (R9), 2001