AHH-1細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
數(shù)量： 大量
細(xì)胞形態(tài)： **樣
年限： 33 years
細(xì)胞類型： B**細(xì)胞
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
器官來(lái)源： 外周血
ATCC Number： CRL-8146?
Designations: AHH-1
Depositors: Massachusetts Institute of Technology
Biosafety Level: 2 [Cells Contain HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
AHH-1細(xì)胞Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Cell Type: B lymphocyte;
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DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10
D13S317: 11,13
D16S539: 10
D5S818: 12,13
AHH-1細(xì)胞D7S820: 10,11
THO1: 6,9.3
TPOX: 8,9
vWA: 18,19
Cytogenetic Analysis: near diploid
Age: 33 years
Gender: male
Ethnicity: AHH-1細(xì)胞Caucasian
Comments: The cells have a high level of oxidative activity, and and can be used in mutagenesis assays for substances that are metabolized into mutagenic products. They are are sensitive to killing by benzo[a]pyrene. The cells carry an endogenous Epstein-Barr virus (EBV) genome and are EBNA positive.
Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, and 1.0 mM sodium pyruvate, 90%; horse serum, 10%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Growth Conditions: The cells grow as floating aggregates of large cell clusters.
Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: culture medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Doubling Time: 16 to 19 hrs
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
parental cell line:AHH-1細(xì)胞ATCC CCL-156
derivative:ATCC CRL-10575
References: 21904: Crespi CL, Thilly WG. Mutation assays involving blood cells that metabolize toxic substances. US Patent 4,532,204 dated Jul 30 1985
