8E5 [derivative of CEM]細(xì)胞
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
細(xì)胞形態(tài)： **樣
細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 人
數(shù)量： 大量
ATCC Number： CRL-8993?
相關(guān)**： 白血病
年限： 4 years
8E5 [derivative of CEM]細(xì)胞器官來(lái)源： 外周血
Designations: 8E5 [derivative of CEM]
Depositors: The United States of America
Biosafety Level: 2 [Cells Contain RETROVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: acute lymphoblastic leukemia
Cell Type: lymphoblasthuman immunodeficiency virus (HIV) positive
Cellular Products: 8E5 [derivative of CEM]細(xì)胞most human immunodeficiency virus (HIV, HTLV III, LAV) structural proteins
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Receptors: transferrin
Antigen Expression: CD4 +; CD5 +
Age: 4 years
Gender: female
Ethnicity: Caucasian
Comments: The cells contain a single defective proviral genome of HTLV III (HIV, LAV).
All of the major structural protein are constitutively expressed with the exception of the p64 and p34 proteins.
No infectious virus is produced; however, co-cultivation with Leu-3 + cells leads to syncytia formation.
The cells have been cured of a mycoplasma contamination.
Propagation: 8E5 [derivative of CEM]細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Cultures can be maintained by addition or replacement of medium. Establish new cultures at 2 X 10 exp5 viable cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6.
Medium Renewal: Add fresh medium (20% to 30% by volume) every 2 to 3 days
Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
parental cell line:ATCC CCL-119
References: 3870: Powell DM, et al. 8E5 [derivative of CEM]細(xì)胞Cell line producing AIDS viral antigens without producing infectious virus particles. US Patent 4,752,565 dated Jun 21 1988
