PC-12 Adh細胞

細胞形態(tài)： 多邊形

運輸方式： 凍存運輸

是否是腫瘤細胞： 0

物種來源： 大鼠

生長狀態(tài)： 貼壁生長

數量： 大量

ATCC Number： CRL-1721.1?

相關**： 其他**

器官來源： 腎上腺

Designations: PC-12 Adh

Depositors: B Patterson

PC-12 Adh細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, small clusters

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: polygonal

Source: Organ: adrenal gland

Disease: pheochromocytoma

Cellular Products: catecholamines; dopamine; norepinephrine [1163]

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Applications: transfection host

Tumorigenic: Yes

Cytogenetic Analysis: 40 chromosomes; 38 autosomes plus XY [1163]

Gender: PC-12 Adh細胞male

Comments: The PC-12 cell line was derived from a transplantable rat pheochromocytoma. The cells do not synthesize epinephrine. This adherent variant (PC-12 Adh) has been adapted to Corning CellBIND&reg flasks to improve cell attachment.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Volumes used for this protocol are for a 75cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard old culture medium.

Pipet 10 ml fresh medium over the cell sheet and scrape.

Aspirate cells with a small bore pipette to break up clusters.

Add appropriate aliquots of the cell suspension to new Corning CellBIND&reg 75 cm2 flask with 15 ml fresh growth medium. Seed flask at 1.0 x 10(4) to 3.0x 10(4) viable cells / cm2 or use subcultivation ratio of 1:3 twice weekly.Subculture when cell density reaches between 1.0x 1 0(5) to 2.0x 10(5) viable cells / cm2.

Place culture vessels in incubator at 37?C.

Medium Renewal: Every 2 to 3 days

Preservation:PC-12 Adh細胞Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Doubling Time: 48 hrs

Related Products: Parent cell line: CRL-1721, PC-12

Recommended medium (without the additional supplements or serum described under ATCC Medium): ATCC 30-2004

recommended serum: ATCC 30-2020

recommended serum: ATCC 30-2040

References: 1162: Levi A, et al. Molecular cloning of a gene sequence regulated by nerve growth factor. Science 229: 393-395, 1985. PubMed: 3839317

1163: Greene LA, Tischler AS. Establishment of a noradrenergic clonal line of rat adrenal pheochromocytoma cells which respond to nerve growth factor. Proc. Natl. Acad. Sci. USA 73: 2424-2428, 1976. PubMed: 1065897

22344: Biocca S, et al. A macromolecular structure favouring microtubule assembly in NGF- differentiated pheochromocytoma cells (PC12). EMBO J. 2: 643-648, 1983. PubMed: 6641712

33014: Weber E, et al. PC-12 Adh細胞Distinct functional properties of Rab3A and Rab3B in PC12 neuroendocrine cells. J. Biol. Chem. 271: 6963-6971, 1996. PubMed: 8636125