AtT-20細(xì)胞

品系： LAF1

ATCC Number： CCL-89?

相關(guān)**： 腫瘤

數(shù)量： 大量

器官來(lái)源： 垂體

運(yùn)輸方式： 凍存運(yùn)輸

細(xì)胞形態(tài)： 其他

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

Designations: AtT-20

Depositors: G Sato

AtT-20細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Mus musculus

Morphology: small rounded cells

AtT-20細(xì)胞

Source: Organ: pituitary

Strain: LAF1

Disease: tumor

Cellular Products: adrenocorticotropic hormone (ACTH)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: October, 1966

Applications: transfection host

Virus Susceptibility: AtT-20細(xì)胞Vesicular stomatitis virus

Human poliovirus 1

Comments: Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 2.5%; horse serum to a final concentration of 15%.

Temperature: 37.0°C

Growth Conditions: The cells do not attach but aggregate to form clusters; the clusters are viable.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Allow clusters to settle and remove all but 5 to 10 ml of medium. Gently transfer the clusters to new flasks and add fresh medium. Proliferation is enhanced by incubation on a shaking platform.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Preservation: Freeze medium: culture medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020

recommended serum:ATCC 30-2040

derivative:AtT-20細(xì)胞ATCC CRL-1795

References: 23251: Buonassisi V, et al. Hormone-producing cultures of adrenal and pituitary tumor origin. Proc. Natl. Acad. Sci. USA 48: 1184-1190, 1962. PubMed: 13874682

26257: Furth J, et al. ACTH secreting transplantable pituitary tumors. Proc. Soc. Exp. Biol. Med. 84: 253-254, 1953. PubMed: 13121002

28712: Tabernero C, et al. Identification of an RNA sequence within an intracisternal-A particle element able to replace Rev-mediated posttranscriptional regulation of human immunodeficiency virus type 1. J. Virol. 71: 95-101, 1997. PubMed: 8985327

33043: Gamby C, et al. Growth-associated protein-43 (GAP-43) facilitates peptide hormone secretion in mouse anterior pituitary AtT-20 cells. J. Biol. Chem. 271: 10023-10028, 1996. PubMed: 8626556