145-2C11細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-1975?

數(shù)量： 大量

細(xì)胞形態(tài)： **樣

**類型： IgG , IgG

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

是否是腫瘤細(xì)胞： 0

物種來源： 倉鼠

Designations: 145-2C11

Depositors: JA Bluestone

Isotype: IgG , IgG

Biosafety Level: 1

Shipped: frozen

145-2C11細(xì)胞Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Cricetulus migratorius (B cell); Mus musculus (myeloma) deposited as hamster, Armenian (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against mouse CD3

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Applications: 145-2C11細(xì)胞It reacts with all mature T cells and can both activate and inhibit T cell function.

The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.

The antibody reacts with the murine T cell receptor (CD3 - T3) complex.

Comments: Animals were immunized with the BM10-37 mouse cytotoxic T lymphocyte (CTL) cell clone (anti H-2 Kb).

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The antibody reacts with the murine T cell receptor (CD3 - T3) complex.

The antibody is specific for a 25000 dalton protein component (CD3 epsilon) of the antigen specific T cell receptor.

145-2C11細(xì)胞It reacts with all mature T cells and can both activate and inhibit T cell function.

The antibody does not react with peripheral blood lymphocytes from rats, rabbits, miniature swine or hamsters.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Medium Renewal: Every 2 to 3 days

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 23298: Leo O, et al. Identification of a monoclonal antibody specific for a murine T3 polypeptide. Proc. Natl. Acad. Sci. USA 84: 1374-1378, 1987. PubMed: 2950524

28992: Kayagaki N, et al. 145-2C11細(xì)胞Polymorphism of murine Fas ligand that affects the biological activity. Proc. Natl. Acad. Sci. USA 94: 3914-3919, 1997. PubMed: 9108079