91MS441細(xì)胞

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

細(xì)胞形態(tài)： **樣

**類型： IgG1; kappa light chain

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 小鼠

ATCC Number： HB-11301?

Designations: 91MS441

Depositors: University of Massachusetts

Isotype: IgG1; kappa light chain

91MS441細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

91MS441細(xì)胞Cellular Products: immunoglobulin; monoclonal antibody; against an antibody to a chlamydia genus antigen (anti-idiotypic antibody)

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Comments: Animals were immunized with 89MS30 (GLXA-Ab1) IgG.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

Propagation: 91MS441細(xì)胞ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and RPMI 1640 medium with 3 mM L-glutamine and 4.5 g/L glucose, 90%; fetal bovine serum, 10%

Temperature: 37.0°C

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 to 2 X 10 exp5 viable cells/ml.

Maintain cell density between 1 X 10 exp5 and 1 X 10 exp6 viable cells/ml.

Preservation: culture medium 95%; DMSO, 5%

Related Products: recommended serum:ATCC 30-2020

References: 40475: MacDonald B, et al. 91MS441細(xì)胞Oral vaccine comprising anti-idiotypic antibody to chlamydia glycolipid exoantigen and process. US Patent 5,656,271 dated Aug 12 1997