Clone 15 HL-60細(xì)胞
器官來源： 外周血
生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)
ATCC Number： CRL-1964?
相關(guān)**： 其他**
年限： 36 years
運(yùn)輸方式： 凍存運(yùn)輸
是否是腫瘤細(xì)胞： 0
物種來源： 人
細(xì)胞形態(tài)： **樣
數(shù)量： 大量
Designations: Clone 15 HL-60
Depositors: S Fischkoff
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Clone 15 HL-60細(xì)胞Growth Properties: suspension
Organism: Homo sapiens
Morphology: lymphoblast
Source: Organ: peripheral blood
Disease: acute promyelocytic leukemia
Cellular Products: eosinophil granule proteins: eosinophil major basic protein (MBP), eosinophil peroxidase (EPO), eosinophil cationic protein (ECP) and eosinophil derived neurotoxin (EDN)
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Isolation: Isolation date: 1984
Receptors: complement, expressed
Fc, expressed
interleukin 5 (IL-5), expressed
Reverse Transcript: negative
DNA Profile (STR): Amelogenin: X
CSF1PO: 13,14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7,8
TPOX: 8,11
vWA: 16
Age: 36 years
Gender: female
Ethnicity: Caucasian
Comments: Clone 15 HL-60細(xì)胞Clone 15 was established in 1984 from a clone of HL-60 (ATCC CCL-240) that had been grown at an elevated pH (pH 7.6 to 7.8) for 2 months.
This clone, picked from soft agar, was selected for its ability to undergo eosinophilic differentiation when treated with butyric acid.
Initially, 80% of the treated cells will differentiate as eosinophils.
However, with continued passage the line will gradually exhibit a modest degree of reversion such that a higher percentage of the cells will exhibit neutrophilic differentiation.
The cells express IL-5 receptors and eosinophil granule proteins, and exhibit chemotaxis to eosinophil specific eosinophilotactins.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Clone 15 HL-60細(xì)胞Cultures can be maintained by addition or replacement of medium. Start new cultures at 2 X 10(5) viable cells/ml and subculture at 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References: 22787: Fischkoff SA. Graded increase in probability of eosinophilic differentiation of HL-60 promyelocytic leukemia cells induced by culture under alkaline conditions.Clone 15 HL-60細(xì)胞 Leuk. Res. 12: 679-696, 1988. PubMed: 3184985
