Mino細(xì)胞

年限： 64

組織來(lái)源： Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

器官來(lái)源： 外周血

生長(zhǎng)狀態(tài)： 懸浮生長(zhǎng)

ATCC Number： CRL-3000?

相關(guān)**： 其他**

細(xì)胞形態(tài)： **樣

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

細(xì)胞類型： 其他細(xì)胞類型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

Designations: Mino

Mino細(xì)胞Depositors: R.J.Ford

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens

Morphology: lymphoblast

Characterization Data

Source: Organ: peripheral blood

Tissue: Mantle cell lymphoma (B cell non-Hodgkin's lymphoma)

Cell type: lymphoblast; cytogenetic abnormality

Disease: Mantle Cell Lymphoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561].

Antigen Expression: CD3-, CD5+, CD10-, CD19+, CD20+, CD23- (verified at ATCC )

DNA Profile (STR): Mino細(xì)胞Amelogenin: X,Y

CSF1PO: 9,11

D13S317: 12

D16S539: 11,12

D5S818: 11,12

D7S820: 10,11

THO1: 9.3

TPOX: 8,11

vWA: 14,17

Cytogenetic Analysis: Modal chromosome number: 74. This is a hypertriploid male cell line, del(6)(q16), t(8;11)(q22;q23), add(11)(q25), add(13)(q34), add(14)(q32). Most cells contain the t(11;14) derivative chromosome, detected by FISH with whole chromosome paint probes.

Age: 64

Gender: male

Ethnicity: Caucasian

Comments: The cells are large, growing singly and in small clumps in vitro. The immunophenotype by flow cytometry is compatible with MCL [PubMed: 12127561].Western blots show expression of cyclin D1 but no detectable cyclin D2 and cyclin D3; the retinoblastoma protein is predominantly phosphorylated. There is expression of tumor suppressor gene products including p53, p16 (INK4a), and p21(WAF1) [PubMed: 12127561].

Propagation: Mino細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 15%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 6 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 1.5 X 10(6) cells/ml. Subcultivation Ratio: 1:5 to 1:10.

Preservation: 90% FBS; 10% (v/v) DMSO

Doubling Time: about 72 hours

References: 92889: Lai R, et al. Establishment and characterization of a new mantle cell lymphoma cell line, Mino. Leuk. Res. 26: 849-855, 2002. PubMed: 12127561

92890: Marzec M, et al. Mantle cell lymphoma cells express predominantly cyclin D1a isoform and are highly sensitive to selective inhibition of CDK4 kinase activity. Blood 108: 1744-1750, 2006. PubMed: 16690963

92895: Salaverria I, et al. Mino細(xì)胞Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365