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產(chǎn)品資料

DSN細(xì)胞

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產(chǎn)品名稱: DSN細(xì)胞
產(chǎn)品型號(hào): DSN
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無(wú)相關(guān)文檔

簡(jiǎn)單介紹

DSN細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o(wú)菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無(wú)菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來(lái)源和培養(yǎng)基配制是減低污染之*好方法。DSN細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長(zhǎng)密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


DSN細(xì)胞  的詳細(xì)介紹

DSN細(xì)胞


ATCC Number: CRL-9939?

相關(guān)**: 骨肉瘤

年限: 11 years

數(shù)量: 大量

細(xì)胞形態(tài): 上皮樣

品系: poodle

運(yùn)輸方式: 凍存運(yùn)輸

生長(zhǎng)狀態(tài): 貼壁生長(zhǎng)

器官來(lái)源: 骨

是否是腫瘤細(xì)胞: 0

物種來(lái)源: 狗

Designations: DSN

Depositors: Wisconsin Alumni Res. Fndn.

DSN細(xì)胞Biosafety Level: 2 [Cells contain CMV, SNV, SV-40 viral DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Canis familiaris

Morphology: epithelial


Source: Strain: poodle

Organ: bone

Disease: osteosarcoma

Derived from metastatic site: lung

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. DSN細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.

Virus Susceptibility: spleen necrosis virus (SNV)

Age: 11 years

Gender: female

Comments: The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.

The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.

Propagation: DSN細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended

Medium Renewal: Every 2 to 3 days

Remove medium, add fresh 0.25% trypsin-0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 21998: DSN細(xì)胞Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992


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