DSN細(xì)胞
ATCC Number： CRL-9939?
相關(guān)**： 骨肉瘤
年限： 11 years
數(shù)量： 大量
細(xì)胞形態(tài)： 上皮樣
品系： poodle
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
器官來(lái)源： 骨
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 狗
Designations: DSN
Depositors: Wisconsin Alumni Res. Fndn.
DSN細(xì)胞Biosafety Level: 2 [Cells contain CMV, SNV, SV-40 viral DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology: epithelial
Source: Strain: poodle
Organ: bone
Disease: osteosarcoma
Derived from metastatic site: lung
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. DSN細(xì)胞Please click here for information regarding the specific requirements for shipment to your location.
Virus Susceptibility: spleen necrosis virus (SNV)
Age: 11 years
Gender: female
Comments: The DSN cell line was derived from D-17 (see ATCC CCL-183) cells by transfecting with plasmids containing the gag-pol and the env genes of spleen necrosis virus.
The line is for use as a helper cell for propagating replication incompetent vectors derived from the SNV retrovirus.
Propagation: DSN細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:12 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add fresh 0.25% trypsin-0.02% EDTA for several minutes, remove trypsin and let the culture sit at 37C for 10 to 15 minutes. Add fresh medium, aspirate and dispense into new flasks. Subculture every 4 to 6 days.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21998: DSN細(xì)胞Temin HM, Dougherty JP. Recombination resistant retroviral helper cell and products produced thereby. US Patent 5,124,263 dated Jun 23 1992
