G-7細胞
品系： Swiss
數(shù)量： 大量
組織來源： skeletal muscle
ATCC Number： CRL-1447?
細胞形態(tài)： 成肌細胞
生長狀態(tài)： 貼壁生長
細胞類型： 其他細胞類型
年限： fetus fetus
是否是腫瘤細胞： 0
物種來源： 小鼠
運輸方式： 凍存運輸
Designations: G-7
Depositors: J Peacock
G-7細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: myoblast
Source: Strain: Swiss
Tissue: skeletal muscle
Cell Type: myoblast myoblast;
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Tumorigenic: Yes
Cytogenetic Analysis: modal number = 40
Age: fetus fetus
Comments: G-7細胞When confluent, the cells fuse to form multinucleated myotubes.
They are slightly responsive to acetylcholine.
The myoblastic component of the population will rapidly become depleted if the cells are allowed to become confluent, since more extensive fusion will take place.
To avoid this, the cells should be subcultured before they become confluent, and they should be recloned periodically with selection for myoblastic clones.
Propagation: ATCC complete growth medium: Dulbecco's modified Eagle's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate and 4.5 g/L glucose, 80%; horse serum, 10%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, add fresh 0.25% trypsin, 0.53mM EDTA solution for 2 to 5 minutes, remove trypsin, add fresh medium, aspirate and dispense into new Bovine Collagen type l (0.03 mg/ml) coated flasks. Differentiation is enhanced by reducing the sera to 2% each.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Doubling Time: 17 to 19 hrs
References: 1063: Christian CN, et al. G-7細胞Synapse formation between two clonal cell lines. Science 196: 995-998, 1977. PubMed: 193191
