Hs 578Bst細胞
器官來源： **
細胞形態(tài)： 成纖維樣
ATCC Number： HTB-125?
數(shù)量： 大量
是否是腫瘤細胞： 0
物種來源： 人
運輸方式： 凍存運輸
生長狀態(tài)： 貼壁生長
年限： 74 years
Designations: Hs 578Bst
Hs 578Bst細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: mammary gland; breast
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Receptors: epidermal growth factor (EGF)
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,13
D13S317: 11,13
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8
vWA: 17
Cytogenetic Analysis: modal number = 46; range = 42 to 48..
This is a diploid human cell line with 46,XX karyotype.Hs 578Bst細胞 Polyploidy occurred at 6.9%. Both X chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.
Isoenzymes: AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 1
Me-2, 0
PGM1, 1
PGM3, 1
Age: 74 years
Gender: female
Ethnicity: Caucasian
Comments: Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126).
Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo.
No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected.
Note: A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximately 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.
Propagation: Hs 578Bst細胞ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X
tumor cell line established from the same patient:ATCC HTB-126
References: 1119: Hackett AJ, et al. Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs 578T) and the diploid myoepithelial (Hs 578Bst) cell lines. J. Natl. Cancer Inst. 58: 1795-1806, 1977. PubMed: 864756
21947: Hancock ME, et al. Method for predicting chemosensitivity of anti-cancer drugs. US Patent 4,937,182 dated Jun 26 1990
24344: Stampfer MR, et al. Hs 578Bst細胞Enhanced growth medium and method for culturing human mammary epithelial cells. US Patent 4,423,145 dated Dec 27 1983
