IC-21細胞

運輸方式： 凍存運輸

ATCC Number： TIB-186?

生長狀態(tài)： 貼壁生長

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 小鼠

品系： C57BL/6

數(shù)量： 大量

Designations: IC-21

Depositors: WS Walker

Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]

Shipped: frozen

Medium & Serum: See Propagation

IC-21細胞Growth Properties: adherent

Organism: Mus musculus

Morphology:

Source: Cell Type: peritoneal macrophage; SV40 transformed

Strain: C57BL/6

Cellular Products: lysozyme

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: Biological response [92560]

transfection host (Roche Transfection Reagents)

Receptors: Fc [1144]

complement (C3) [1231]

Comments: The IC-21 cell line was derived by transformation of normal C57BL/6 mouse peritoneal macrophages with SV40. [22225]

This line shares many properties with normal mouse macrophages and display macrophage specific antigens.

They have phagocytic and cytolytic properties, can lyse tumor targets in vitro and appear to be more differentiated than cells of the P388D1 macrophage line. [1231] [22279]

Trypsin is toxic to this line.

The cells produce large quantities of acid and the medium should be changed frequently.

Tested and found negative for ectromelia virus (mousepox).

Propagation: IC-21細胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: 3 times per week

Rinse the monolayer with 10 to 15 ml of calcium, magnesium free PBS, then add an additional 10 to 15 ml of the same solution.

Let the culture stand for 5 to 10 minutes at room temperature, strike the flask to dislodge cells, add 5 to 7 ml of the cell suspension to a flask containing less than 10 ml of growth medium.

Add additional medium once the cells have attached (one to two days).

Subculture when confluent.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 1144: Walker WS. Separate Fc-receptors for immunoglobulins IgG2a and IgG2b on an established cell line of mouse macrophages. J. Immunol. 116: 911-914, 1976. PubMed: 1254971

1231: Walker WS, Gandour DM. Detection and functional assessment of complement receptors on two murine macrophage-like cell lines. Exp. Cell Res. 129: 15-21, 1980. PubMed: 7428810

1233: Walker WS. Mediation of macrophage cytolytic and phagocytic activities by antibodies of different classes and class-specific Fc-receptors. J. Immunol. 119: 367-373, 1977. PubMed: 886183

22203: Mocarelli P, et al. IC-21細胞A permanent line of macrophages with normal activity in a primary antibody response in vitro. Immunol. Commun. 2: 441-447, 1973. PubMed: 4357034

22225: Mauel J, Defendi V. Infection and transformation of mouse peritoneal macrophages by simian virus 40. J. Exp. Med. 134: 335-350, 1971. PubMed: 4326994

22279: Holden HT, et al. . Fed. Proc. 38: 1093 (abstract 4582), 1979.

22826: Walker WS, Demus A. Antibody-dependent cytolysis of chicken erythrocytes by an in vitro- established line of mouse peritoneal macrophages. J. Immunol. 114: 765-769, 1975. PubMed: 1167563

22967: Singer JA, et al. Interaction of a mouse macrophage cell line with homologous erythrocytes. J. Reticuloendothel. Soc. 31: 489-499, 1982. PubMed: 7120230

32968: Takao S, et al. Role of reactive oxygen metabolites in murine peritoneal macrophage phagocytosis and phagocytic killing. Am. J. Physiol. 271: C1278-C1284, 1996. PubMed: 8897835

58080: Serio C, et al. Macrophage functional heterogeneity: evidence for different antibody-dependent effector cell activities and expression of Fc-receptors among macrophage subpopulations. J. Reticuloendothel. Soc. 25: 197-206, 1979. PubMed: 439098

92560: IC-21細胞Standard Practice for Testing for Biological Responses to Particles in Vitro. West Conshohocken, PA:ASTM International;ASTM Standard Test Method F 1903-98R03.