S16細胞

年限： neonatal

ATCC Number： CRL-2941?

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 上皮樣

運輸方式： 凍存運輸

組織來源： sciatic nerve

細胞類型： 其他細胞類型

是否是腫瘤細胞： 0

物種來源： 褐鼠

數(shù)量： 大量

S16細胞Designations: S16

Depositors: RH Quarles

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus

Morphology: epithelial-like

Source: Tissue: sciatic nerve

Cell type: Schwann cell

Cellular Products: laminin [16172656]

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Isolation: Isolation date: 1989

Antigen Expression: S16細胞Myelin-associated glycoprotein (MAG) [16172655]

Galactocerebroside (GalC) [16172655]

Age: neonatal

Comments: The S16 cell line is derived from a primary culture of Schwann cells that was immortalized by repetitive passaging. High Myelin-associated glycoprotein (MAG) expression was demonstrated by Radioimmunoassay. The S16 cells divide very rapidly, are rounder than normal Schwann cells and elaborate many processes after reaching high density. The cells express galactocerebroside (GalC), sulfatide and P0 glycoprotein, but little or no myelin basic protein. [16172655] [16172656]

In many respects, the S16 and S42 (ATCC CRL-2942) cells, biochemically, resemble Schwann cells at an early stage in their preparation to myelinate and should be useful for investigating the cell biology of MAG and other myelin-related components. [16172656]

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: The culture flasks should be treated with 0.1ml/sq. cm of flask surface area with 15ug/ml poly-L-lysine (Sigma Cat. No. P-9155 or equivalent) for at least 2 hours at 37°C. Remove solution and rinse one time with DPBS and allow flask to air dry uncapped and standing upright in a biological cabinet for about 30 minutes before introducing cells.

Volumes used in this protocol are for 75 sq. cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

S16細胞Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (DPBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 1.0 to 2.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Transfer cell suspension to a centrifuge tube and spin at approximately 125 X g for 5 to 10 minutes. Discard supernatant.

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels. An inoculum of 4 X 10(3) to 6 X 10(4) viable cells/sq. cm is recommended.

Incubate cultures at 37°C. We recommend that you maintain cultures at a cell concentration between 2 X 10(4) and 4 X 10(4) cells/sq. cm.

Subcultivation ratio: A subcultivation ratio of 1:4 to 1:6 twice weekly is recommended.

Interval: Subculture when cell concentration is between 2 x10(4) and 4x10(4) cells/sq. cm.

Medium renewal: Every 2 to 3 days

Preservation: Freeze medium: complete growth medium, 90%; DMSO, 10%

Storage temperature: liquid nitrogen vapor phase

Doubling Time: approximately 40 hours

Related Products: Recommended medium (without the additional serum described under ATCC Medium): ATCC 30-2002

Recommended serum: S16細胞ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101

Phosphate-buffered saline: ATCC 30-2200

Cell culture tested DMSO: ATCC 4-X

Erythrosin B vital stain solution: ATCC 30-2404

Other ATCC Cell Lines: CRL

Other ATCC Cell Lines: CRL

Other ATCC Cell Lines: CRL

References: 16172655: Goda S, et al. Expression of the myelin-associated glycoprotein in cultures of immortalized Schwann cells. J. Neurochem. 56(4):1354-1361, 1991. PubMed: 1705958

16172656: Toda K, et al. Biochemical and cellular properties of three immortalized Schwann cell lines expressing different levels of the myelin-associated glycoprotein. J. Neurochem. 63(5):1646-1657, 1994. PubMed: 7523597

16172657: Sasagasako N, et al. Myelin gene expression in immortalized Schwann cells: relationship to cell density and proliferation. J. Neurochem. 66(4): 1432-1439, 1996. PubMed: 8627295