TGP52細(xì)胞

品系： Tg(E1a-1-SV40E)Bri18

器官來源： 胰腺

細(xì)胞形態(tài)： 上皮樣

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-2140?

運(yùn)輸方式： 凍存運(yùn)輸

相關(guān)**： 其他**

年限： 26 weeks

是否是腫瘤細(xì)胞： 0

物種來源： 轉(zhuǎn)基因小鼠

Designations: TGP52

TGP52細(xì)胞Depositors: OS Pettengill, D Longnecker

Biosafety Level: 2 [CELLS CONTAIN PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus, transgenic deposited as mouse, transgenic

Morphology: epithelial

Source: Organ: pancreas

Strain: Tg(E1a-1-SV40E)Bri18

Disease: pancreatic islet cell tumor; insulinoma

Cellular Products: somatostatin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: TGP52細(xì)胞Note: These cells are distributed subject to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Dartmouth College. 2.) Any proposed commercial use of these cells must first be negotiated with Office of Technology Transfer, Dartmouth College, Hanover, NH, 03755. Tel: (603) 646-3675. 3.) In all papers reporting any use of these cells, or derived products, a direct reference will be made to the original publications.

Age: 26 weeks

Gender: male

Comments: TGP52 is an epithelial like cell line derived from a pancreatic tumor with islet cell morphology (insulinoma) arising in an ***** male transgenic mouse.

The mouse carried the pseudogene construct composed of elastase-1 promoter linked to the SV40 T antigen.

In culture, TGP52 cells transiently produced insulin in culture and then produced somatostatin.

Current stocks of TGP52 secrete about 9700 pg/ml of somatostatin in 3 days.

Morphologic and differentiation markers of this line appear to fluctuate from time to time in culture.

Propagation: ATCC complete growth medium: A 1:1 mixture of Dulbecco's modified Eagle's medium and Ham's F12 medium, 90%; heat-inactivated fetal bovine serum, 10%

Subculturing: TGP52細(xì)胞Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended

Medium Renewal: Twice per week

Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin. Let the culture sit at room temperature (or at 37C) until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 22323: Pettengill OS, et al. Cell lines derived from pancreatic tumors of Tg(Ela-1-SV40E)Bri18 transgenic mice express somatostatin and T antigen. Carcinogenesis 15: 61-65, 1994. TGP52細(xì)胞PubMed: 7904904