VLN6H2細胞

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

是否是腫瘤細胞： 0

物種來源： 人

運輸方式： 凍存運輸

細胞形態(tài)： **樣

**類型： IgG

ATCC Number： HB-8633?

Designations: VLN6H2

Depositors: Regents of the University of California

Isotype: IgG

Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]

Shipped: frozen

VLN6H2細胞Medium & Serum: See Propagation

Growth Properties: suspension

Organism: human (B cell); human (myeloma)

Morphology: lymphoblast

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against cancer of the vulva

Permits/Forms: VLN6H2細胞In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: The fusion line, CRL-8061, is reported to be EBNA positive.

Lymphocytes were isolated from a regional draining lymph node of a patient with carcinoma of the vulva.

B lymphocytes were fused with UC 729-6 cells (see ATCC CRL-8061).

VLN6H2細胞The antibody recognizes an epitope on a 78000 dalton antigen on A-431 cells and a 66000 dalton antigen on T293H cells.

Propagation: ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine, 90%; fetal bovine serum, 10%

Subculturing: Medium Renewal: Every 2 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 8 X 10 exp5 cells/ml.

References: 21921: Glassy MC, Surh C. VLN6H2細胞Human-human hybrid cell lines that produce antibodies against antigenic determinants on cancer cells. US Patent 4,761,377 dated Aug 2 1988