Detroit 551細胞
ATCC Number： CCL-110?
相關**： 正常
細胞形態(tài)： 成纖維樣
運輸方式： 凍存運輸
器官來源： 皮膚
細胞類型： 成纖維細胞
是否是腫瘤細胞： 0
物種來源： 人
生長狀態(tài)： 貼壁生長
年限： fetus
數(shù)量： 大量
Designations: Detroit 551
Detroit 551細胞Depositors: CS Stulberg
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: fibroblast
Source: Organ: skin
Disease: normal
Cell Type: fibroblast
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Isolation: Isolation date: 1965
Applications: Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Virus Susceptibility: SV40 virus
Human poliovirus 1
Detroit 551細胞Herpes simplex virus
Vesicular stomatitis virus
DNA Profile (STR): Amelogenin: X
CSF1PO: 10,12
D13S317: 11,12
D16S539: 12,13
D5S818: 12,13
D7S820: 10,12
THO1: 9,9.3
TPOX: 8
vWA: 18
Cytogenetic Analysis: normal human female; diploid
Isoenzymes: G6PD, B
Age: fetus
Gender: female
Ethnicity: Caucasian
Comments: The cells have a finite lifespan of about 25 serial passages from the tissue of origin.
They can be transformed by SV40.
Growth of the cells is enhanced by addition of tumor necrosis factor alpha (TNF alpha) to the medium.
Propagation: ATCC complete growth medium: Detroit 551細胞The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.53 mM EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.Add fresh culture medium, aspirate and dispense into new culture flasks.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:5 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 21405: Detroit 551細胞Sugarman BJ, et al. Recombinant human tumor necrosis factor-alpha: effects on proliferation of normal and transformed cells in vitro. Science 230: 943-945, 1985. PubMed: 3933111
