OA3.Ts細胞

細胞形態(tài)： 上皮樣

年限： fetus

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

運輸方式： 凍存運輸

ATCC Number： CRL-6546?

相關**： 正常

器官來源： 睪丸

是否是腫瘤細胞： 0

物種來源： 綿羊

Designations: OA3.Ts

Biosafety Level: 1

OA3.Ts細胞Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Ovis aries

Morphology: epithelial

Source: Organ: testis

Disease: normal

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. OA3.Ts細胞Please click here for information regarding the specific requirements for shipment to your location.

Age: fetus

Gender: male

Comments: Part of the NBL Collection. Unlike other cell lines in the NBL Collection, this item has been fully accessioned by ATCC and is covered by the standard warranty.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; OA3.Ts細胞carbon dioxide (CO2), 5%

Subculturing: Protocol: Volumes used in this protocol are for 75 sq cm flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

Remove and discard culture medium.

Briefly rinse the cell layer with Ca++/Mg++ free Dulbecco's phosphate-buffered saline (D-PBS) or 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.

Add 2.0 to 3.0 ml of complete growth medium and aspirate cells by gently pipetting

Resuspend the cell pellet in fresh growth medium. Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37C.

Preservation: OA3.Ts細胞Freeze medium: complete growth medium, 95%; DMSO, 5%