WR21細(xì)胞

年限： *****

細(xì)胞形態(tài)： 成纖維樣

品系： subline 69-2 (C57BL/6, SJL transgenic)

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2189?

相關(guān)**： 腺癌

器官來源： 其他

生長狀態(tài)： 貼壁生長

數(shù)量： 大量

Designations: WR21

Depositors: LL Nielsen

WR21細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Mus musculus

Morphology: fibroblast

Source: Organ: submandibular salivary gland

Strain: subline 69-2 (C57BL/6, SJL transgenic)

Disease: adenocarcinoma

Cellular Products: vimentin; fibronectin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. WR21細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: These cells are distributed subjected to the following: 1.) This cell line or its products must not be distributed to third parties. Commercial interests are the exclusive property of Schering- Plough Research Institute. 2.) Any proposed commercial use of these cells must first be negotiated with Ms. Georgina Wertman, Schering Corporation, 2000 Galloping Hill Road, K61, Kenilworth, New Jersey, 07033. 3.) In all papers reporting any use of these cells or derived products a direct reference will be made to Nielsen et al, In vivo 8:295-302, 1994.

Tumorigenic: Yes

Oncogene: c-H-ras + (human)

Age: *****

Gender: male

Comments: WR21細(xì)胞WR21 cells were cultured from a salivary tumor taken from an ***** male wap-ras subline 69=2 transgenic mouse in the fall of 1989.

These mice express an activated human c-Ha-ras gene and develop adenocarcinomas arising from the submandibular gland and/or mammary progenitor cells by one year of age.

Subline 69-2 has a random bred genetic background of two inbred mouse strains, C57BL/6 and SJL.

The cells were subcloned in 1990.

It is impossible to ascertain the original cell type with any certainty.

They express fibronectin and vimentin but not cytokeratin or desmin.

WR21 cells have an activated human Ha-ras oncogene on the Y chromosome and express Asp-12 mutant ras protein.

In nude mice with WR21 tumors, approximately 90% had lung metastases by the fifth week of tumor growth, but no tumors metastasized to the salivary gland.

WR21 tumor growth was inhibited by cyclophosphamide, 5-fluorouracil, adriamycin, mitomycin C and actinomycin D but not methotrexate.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended

Medium Renewal: WR21細(xì)胞Twice per week

Remove medium, rinse flask with fresh 0.25% trypsin, 0.02% EDTA and allow the flask to sit at room temperature until the cells detach. Add fresh medium, aspirate and dispense into new flasks.

Preservation: Culture medium, 95%; DMSO, 5%

References: 24390: Nielsen LL, et al. Development of a nude mouse model of ras-mediated neoplasia using WR21 cells from a transgenic mouse salivary tumor. In Vivo 8: 295-302, 1994. PubMed: 7803707