I 9.2細胞

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

數(shù)量： 大量

細胞形態(tài)： **樣

細胞類型： T**細胞

ATCC Number： CRL-2571?

相關(guān)**： 白血病

是否是腫瘤細胞： 0

物種來源： 人

Designations: I 9.2

Depositors: J Blenis

I 9.2細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Homo sapiens deposited as human

Morphology: lymphoblast

Source: Disease: acute T cell leukemia

Cell Type: T lymphocyte;

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DNA Profile (STR): Amelogenin: X

CSF1PO: 11,12

D13S317: 8,11

D16S539: 11

D5S818: 9

D7S820: 8,9.2

THO1: 6,9.3

TPOX: 8,10

vWA: 18

I 9.2細胞Gender: male

Comments: The I 9.2 cell line is a caspase-8 mutant of the wild-type Jurkat cell line A3 (see ATCC CRL-2570).

Wild-type A3 cells were made neomycin resistant and treated with three cycles of exposure to the frameshifting mutagen ICR-191 to isolate clones harboring recessive mutations that were resistant to killing by Fas antibody. [51527]

ICR-191 treated clones were serially diluted in 96-well plates in the presence of Fas Antibody for 3 to 5 weeks. [51529]

Two of these ICR-191 treated clones have been deposited at the ATCC . They are I 9.2 (ATCC CRL-2571), a clone with a mutation in the cysteine protease caspase-8/FLICE and I 2.1 (ATCC CRL-2572), a clone with a mutation in the adaptor FADD.

The I 9.2 cell line is functionally defective for caspase-8 and the I 2.1 cell line is functionally defective for FADD.

The I 9.2 cell line and the I 2.1 cell lines are completely resistant to Fas-induced apoptosis. [51527] [51529]

Complementation of I 9.2 cell line with wild-type caspase-8 restored Fas-mediated apoptosis. [51527]

The I 9.2 cell line is severely deficient in cell death induced by TNF-alpha and is partially deficient in cell death induced by ultraviolet irradiation, adriamycin and etoposide. [51527]

This cell line can be used to study the role of caspase-8 in apoptotic signaling pathways in the absence of overexpression.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: I 9.2細胞Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 to 5 X 10(5) viable cells/ml. Maintain cultures at cell concentrations between 2 X 10(5) and 2 X 10(6) viable cells/ml.

Medium Renewal: Add fresh medium every 2 to 3 days (depending on cell density)

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

parental cell line:ATCC CRL-2570

References: 51527: Juo P, et al. Essential requirement for caspase-8/FLICE in the initiation of the Fas-induced apoptotic cascade. Curr. Biol. 8: 1001-1008, 1998. PubMed: 9740801

51529: Juo P, et al. FADD is required for multiple signaling events downstream of the receptor Fas. Cell Growth Differ. 10: 797-804, 1999. PubMed: 10616904

51530: Juo P, et al. Fas activation of the p38 mitogen-activated protein kinase signalling pathway requires ICE/CED-3 family proteases. Mol. Cell. Biol. 17: 24-35, 1997. PubMed: 8972182

51532: Ding HF, et al. I 9.2細胞Essential role for caspase-8 in transcription-independent apoptosis triggered by p53. J. Biol. Chem. 275: 38905-38911, 2000. PubMed: 10988287