CCD-1137Sk細(xì)胞

細(xì)胞類型： 成纖維細(xì)胞

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

運(yùn)輸方式： 凍存運(yùn)輸

ATCC Number： CRL-2703?

器官來(lái)源： 皮膚

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

年限： newborn

細(xì)胞形態(tài)： 成纖維樣

數(shù)量： 大量

CCD-1137Sk細(xì)胞Designations: CCD-1137Sk

Depositors: F.Haq, ATCC

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens deposited as human

Morphology: fibroblast

CCD-1137Sk細(xì)胞

Source: Organ: skin; foreskin

Cell Type: fibroblast

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Age: CCD-1137Sk細(xì)胞newborn

Gender: male

Comments: The line was established from skin taken from normal foreskin.

Cells senesce after approximately 60 population doublings.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:6 is recommended

Medium Renewal: CCD-1137Sk細(xì)胞Every 2 to 3 days

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Add fresh culture medium, aspirate and dispense into new culture flasks.

Preservation: culture medium 95%; DMSO, 5%

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020