30-5-7S細胞

細胞形態(tài)： **樣

**類型： IgG2a; kappa light chain

ATCC Number： HB-31?

是否是腫瘤細胞： 0

物種來源： 小鼠

生長狀態(tài)： 懸浮生長

運輸方式： 凍存運輸

數(shù)量： 大量

Designations: 30-5-7S

Depositors: DH Sachs, K Ozato

30-5-7S細胞Isotype: IgG2a; kappa light chain

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma) deposited as mouse (B cell); mouse (myeloma)

Morphology: lymphoblast

30-5-7S細胞

Source: Cell Type: hybridoma: B lymphocyte;

Cellular Products: immunoglobulin; monoclonal antibody; against L d, D q, L q an d L b; cytotoxic

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Comments: Animals were immunized with spleen cells and tail skin from BALB/c mice.

Spleen cells were fused with Sp2/0-Ag14 myeloma cells.

The reactivity corresponds to the H2.65 serological specificity.

30-5-7S細胞The antibody is cytotoxic.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:

fetal bovine serum to a final concentration of 10%

1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.

Subculturing: Medium Renewal: Every 2 to 3 days

Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 1 X 10 exp5 and 1 X 10 exp6 cells/ml.

Related Products: Recommended medium30-5-7S細胞 (without the additional supplements or serum described under ATCC Medium):ATCC 46-X

References: 948: Ozato K, et al. Monoclonal antibodies to mouse MHC antigens. II. Antibodies to the H-2 Ld antigen, the products of a third polymorphic locus of the mouse major histocompatibility complex. J. Immunol. 125: 2473-2477, 1980. PubMed: 7191868