Sol8細胞
品系： C3H
組織來源： skeletal muscle
ATCC Number： CRL-2174?
運輸方式： 凍存運輸
數(shù)量： 大量
細胞類型： 其他細胞類型
生長狀態(tài)： 貼壁生長
細胞形態(tài)： 成纖維樣
是否是腫瘤細胞： 0
物種來源： 小鼠
年限： 4 weeks
Sol8細胞Designations: Sol8
Depositors: R Bassel-Duby
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Mus musculus
Morphology: fibroblast
Sol8細胞
Source: Tissue: skeletal muscle
Strain: C3H
Cell Type: myoblast myoblast;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Sol8細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 4 weeks
Comments: Sol8 is a myogenic cell line isolated by Daubas et al. from primary cultures of soleus muscle taken from the leg of a normal C3H mouse.
The cells were split two times and then cloned by limiting dilution.
Sol8 cells will differentiate into myotubes with serum deprivation.
They have a phenotype similar to slow twitch fibers.
Note: do not allow the cells to become confluent.
The myoblast component of the population will rapidly become depleted if the culture becomes confluent.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:8 to 1:12 is recommended
Medium Renewal: 2 to 3 times per week
Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.
Add fresh trypsin solution (1 to 2 ml) and let the culture sit at room temperature (or at 37C) until the cells detach.
Add fresh medium, aspirate and dispense into new flasks.
Subculture at 50% of confluence.
Preservation: Sol8細胞Culture medium, 95%; DMSO, 5%
Doubling Time: 16 to 18 hrs
References: 23248: Daubas P, et al. Functional activity of the two promoters of the myosin alkali light chain gene in primary muscle cell cultures: comparison with other muscle gene promoters and other culture systems. Nucleic Acids Res. 16: 1251-1271, 1988. PubMed: 2894633
