Ran De細(xì)胞

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

ATCC Number： CRL-1287?

器官來(lái)源： 皮膚

相關(guān)**： 成骨不全癥

細(xì)胞形態(tài)： 成纖維樣

年限： 9 years

數(shù)量： 大量

Ran De細(xì)胞Designations: Ran De

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: fibroblast

Ran De細(xì)胞

Source: Organ: skin

Disease: osteogenesis imperfecta (congenita)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Age: 9 years

Gender: male

Comments: Ran De細(xì)胞The tissue donor suffered from osteogenesis imperfecta (congenita).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Remove spent medium, add fresh 0.25% trypsin for 3 to 5 minutes, remove trypsin and let the culture sit at 37C for 10 to 20 minutes. Add fresh medium, aspirate and dispense into new flasks. Ran De細(xì)胞Subculture every 8 to 10 days.