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產(chǎn)品資料

YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞

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產(chǎn)品名稱: YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞
產(chǎn)品型號(hào): YB2/0 [YB2/3HL.P2.G11.16Ag.20]
產(chǎn)品展商: HZbscience
產(chǎn)品文檔: 無相關(guān)文檔

簡單介紹

YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞應(yīng)如何避免細(xì)胞污染,細(xì)胞污染的種類可分成**、酵母菌、霉菌、病毒和霉?jié){菌。主要的污染原因?yàn)闊o菌操作技術(shù)不當(dāng)、操作室環(huán)境不佳、污染之血清和污染之細(xì)胞等。嚴(yán)格之無菌操作技術(shù)、清潔的環(huán)境、與品質(zhì)良好之細(xì)胞來源和培養(yǎng)基配制是減低污染之*好方法。YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞何時(shí)須更換培養(yǎng)基?視細(xì)胞生長密度而定,或遵照細(xì)胞株基本數(shù)據(jù)上之更換時(shí)間,按時(shí)更換培養(yǎng)基即可。


YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞  的詳細(xì)介紹

YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞

細(xì)胞形態(tài): **樣

運(yùn)輸方式: 凍存運(yùn)輸

生長狀態(tài): 懸浮生長

是否是腫瘤細(xì)胞: 0

物種來源: 大鼠

ATCC Number: CRL-1662?

數(shù)量: 大量

Designations: YB2/0 [YB2/3HL.P2.G11.16Ag.20]

Depositors: C Milstein

YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: lymphoblast


Source: Cell Type: hybridoma: B lymphoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞This line was produced by fusing Y3/Ag1.2.3 (ATCC CRL-1631) cells with spleen cells from an AO strain rat.

The cells do not secrete either heavy or light immunoglobulin chains.

The cells are resistant to 8-azaguanine, are HAT sensitive and can be used as a fusion partner for rat B cells to make rat - rat hybridomas.

Tested and found negative for ectromelia virus (mousepox).

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing: YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞Medium Renewal: Every 2 to 3 days

Cultures are maintained by addition or replacement of fresh medium. Start new cultures at 1 X 10 exp5 viable cells/ml and maintain at or below 1 X 10 exp6 cells/ml.

References: 1094: Kilmartin JV, et al. Rat monoclonal antitubulin antibodies derived by using a new nonsecreting rat cell line. J. Cell Biol. 93: 576-582, 1982. PubMed: 6811596

22292: . . YB2/0 [YB2/3HL.P2.G11.16Ag.20]細(xì)胞Methods Enzymol. 73B: 1-46, 1981.

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