FAT 7細胞

運輸方式： 凍存運輸

年限： *****

ATCC Number： CRL-2109?

數(shù)量： 大量

相關**： 鱗狀細胞癌

組織來源： nasal

生長狀態(tài)： 貼壁生長

細胞形態(tài)： 上皮樣

是否是腫瘤細胞： 0

物種來源： 大鼠

Designations: FAT 7

Depositors: E Bermudez

FAT 7細胞Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Tissue: nasal

Disease: squamous cell carcinoma

Cellular Products: transforming growth factor alpha (TGF alpha)

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Receptors: epidermal growth factor (EGF)

Tumorigenic: FAT 7細胞Yes

Age: *****

Gender: male

Comments: The FAT 7 line was established from a nasal squamous cell carcinoma induced by formaldehyde inhalation in an ***** male rat.

The cells contain a point mutation in the conserved V region of the p53 gene at codon 271 (CGT --> CAT transversion).

Propagation: ATCC complete growth medium: Ham's F12K medium with 0.01 mg/ml insulin, 250 ng/ml hydrocortisone and 0.0025 mg/ml transferrin, 90%; fetal bovine serum, 10%

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Twice per week

FAT 7細胞Remove spent medium, add fresh 0.25% trypsin, 0.03% EDTA solution, rinse and remove trypsin.

Add 1 to 2 ml of additional trypsin solution and let the culture sit at room temperature (or at 37C) until the cells detach.

Add fresh medium, aspirate and dispense into new flasks.

Subculture every 6 to 8 days.

Preservation: Culture medium, 95%; DMSO, 5%

Doubling Time: 24 hrs

References: 22639: Bermudez E, et al. Characterization of cell lines derived from formaldehyde-induced nasal tumors in rats. Mol. Carcinog. 9: 193-199, 1994. FAT 7細胞PubMed: 8148052