CCD 841 CoTr細胞
數(shù)量： 大量
運輸方式： 凍存運輸
ATCC Number： CRL-1807?
器官來源： 結腸
細胞類型： SV40轉化細胞
是否是腫瘤細胞： 0
物種來源： 人
年限： 21 weeks gestation
生長狀態(tài)： 貼壁生長
CCD 841 CoTr細胞細胞形態(tài)： 上皮樣
Designations: CCD 841 CoTr
Depositors: A Thompson
Biosafety Level: 2 [Cells contain polyomavirus DNA sequences ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens
Morphology: epithelial
Source: Organ: colon
Cell Type: SV40 transformed
Permits/Forms:CCD 841 CoTr細胞 In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Cytogenetic Analysis: The karyology of this line changed dramatically from the parental, but there are reports in the literature of SV40 causing such cytogenetic instability. STR results have consistently shown that CRL-1807 is 100% related to CRL-1790. This is a pentaploid human cell line, probably of female origin. The modal chromosome number is 113 occurring in 14% of cells; cells with 114 chromosomes occurred also at a high rate (10%). The rate of polyploid cells was 3.4%. Most cells had 12-15 markers. Among these, t(1p18q), del(7)(q22q32), t(6p22q), and del(12)(q26.1) were common to most cells. There were 4-5 X chromosomes per cell. Modal copy number per cell for autosomes was 5; N4, N8, N10, N16, N19 and N22 had relatively wide ranges of number of copies per cell. Karyotypes are very heterogeneous.
Age: 21 weeks gestation
Gender: female
Comments: CCD 841 CoTr細胞This line was derived from the same tissue sample as CCD 841 CoN (see ATCC CRL-1790) by transformation with a temperature sensitive mutant of SV40.
The cells exhibit the transformed phenotype at the permissive temperature (33C) for the virus.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: bovine calf serum to a final concentration of 10%.
Temperature: 33.0°C
Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:5 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, add 0.25% trypsin, 0.03% EDTA solution and let the culture sit at 37C for 2 minutes.CCD 841 CoTr細胞 Remove the trypsin and incubate the flask at 33C for 5 to 10 minutes or until the cells detach. Add fresh medium, aspirate and dispense into new flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2030
