2E8細(xì)胞

年限： immature

細(xì)胞形態(tài)： **樣

**類型： IgM , IgM

品系： BALB/c.xid

運(yùn)輸方式： 凍存運(yùn)輸

生長狀態(tài)： 懸浮生長

數(shù)量： 大量

器官來源： 骨髓

ATCC Number： TIB-239?

細(xì)胞類型： B**細(xì)胞

是否是腫瘤細(xì)胞： 0

2E8細(xì)胞物種來源： 小鼠

Designations: 2E8

Depositors: PW Kincade

Isotype: IgM , IgM

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus

Morphology: lymphoblast

Source: Organ: bone marrow

Strain: BALB/c.xid

Cell Type: 2E8細(xì)胞B lymphocyte;

Cellular Products: immunoglobulin (surface IgM)

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Restrictions: 1. This hybridoma is provided for research purposes only. Neither the cell line nor the antibody derived from it may be sold or used for commercial purposes. Nor can the cells be distributed to third parties for purpose of sale, or producing for sale, cells or their products. The cells are provided as a service to the research community. They are provided without warranty or merchantability of fitness for a particular purpose or any other warranty, express or implied. 2. Any proposed commercial use of these cells or antibody produced from them must first be negotiated with the Oklahoma Medical Research Foundation, 825 N.E. 13th, Oklahoma City, OK 73104. Telephone (405) 271-7905.

Receptors: interleukin 7 (IL-7)

Antigen Expression: H-2 d; Ia d; BP-1

Age:2E8細(xì)胞 immature

Comments: The 2E8 line is dependent on IL-7, and can be used for detecting and quantifying IL-7.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium with 4 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 0.05 mM 2-mercaptoethanol, 1 ng/ml mouse interleukin-7 and 20% fetal bovine serum

Temperature: 37.0°C

Subculturing: Protocol: Every 2 to 3 days, the cells must be diluted without centrifugation at a concentration of 5 X 10(5) viable cells/ml by adding fresh medium to the culture.

.Mouse IL-7 can be obtained from several commercial sources including Upstate Biotechnology, Inc., Lake Placid, NY (1-800-548-7853).

The cells do not grow as well when human IL-7 is substituted for mouse IL-7.

Medium Renewal: 2E8細(xì)胞Every 2 to 4 days

Preservation: Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005

recommended serum:ATCC 30-2020

References: 22476: Pietrangeli CE, et al. Stromal cell lines which support lymphocyte growth: characterization, sensitivity to radiation and responsiveness to growth factors. Eur. J. Immunol. 18: 863-872, 1988. PubMed: 3260182

22740: Ishihara K, et al. Stromal-cell and cytokine-dependent lymphocyte clones which span the pre-B- to B-cell transition. Dev. Immunol. 1: 149-161, 1991. 2E8細(xì)胞PubMed: 1821694