CV-1/EBNA-1細(xì)胞
數(shù)量： 大量
器官來(lái)源： 腎臟
ATCC Number： CRL-10478?
相關(guān)**： 正常
運(yùn)輸方式： 凍存運(yùn)輸
生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)
年限： 141 days
是否是腫瘤細(xì)胞： 0
物種來(lái)源： 綠長(zhǎng)尾猴
細(xì)胞形態(tài)： 成纖維樣
Designations: CV-1/EBNA-1
Depositors: Immunex Corporation
CV-1/EBNA-1細(xì)胞Biosafety Level: 2 [CELLS CONTAIN HERPESVIRUS ]
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Cercopithecus aethiops
Morphology: fibroblast
Source: Organ: kidney
Disease: normal
Cellular Products: Epstein-Barr nuclear antigen 1 (EBNA-1)
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications: transfection host [42019]
Virus Susceptibility: CV-1/EBNA-1細(xì)胞poliovirus 1; herpes simplex; Eastern equine encephalitis; Western equine encephalitis; California encephalitis; SV40
Reverse Transcript: negative
Age: 141 days
Gender: male
Comments: Expression of EBNA-1 permits episomal replication of expression vectors such as pDC406 that contain the EBV origin of replication. The cells can be used as host for expression of transfected DNA.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. CV-1/EBNA-1細(xì)胞To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing: Protocol: Remove old medium, add fresh 0.25% trypsin solution for 2 to 3 minutes, remove trypsin and let culture stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: Twice per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: parental cell line:ATCC CCL-70
References: 30208: McMahan CJ, et al. A novel IL-1 receptor, cloned from B cells by mammalian expression, is expressed in many cell types. EMBO J. 10: 2821-2832, 1991. PubMed: 1833184
42019: Gearing DP. CV-1/EBNA-1細(xì)胞Receptor for oncostatin M and leukemia inhibitory factor. US Patent 5,262,522 dated Nov 16 1993
