MAb 951-5-1細(xì)胞

數(shù)量： 大量

ATCC Number： HB-12684?

細(xì)胞形態(tài)： **樣

**類型： IgG1 kappa

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來源： 小鼠

MAb 951-5-1細(xì)胞生長狀態(tài)： 懸浮生長

Designations: MAb 951-5-1

Depositors: The United States of America

Isotype: IgG1 kappa

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: suspension

Organism: Mus musculus (B cell); Mus musculus (myeloma)

Morphology: MAb 951-5-1細(xì)胞lymphoblast

Source: Cell Type: hybridoma:lymphoblast B lymphocyte;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Comments: Animals were immunized with baculovirus-expressed human cytochrome P450 1A2 [U.S. Pat. 6,335,428].Spleen cells were fused ?MAb 26-7-5 (ATCC HB-12681), MAb 951-5-1 (ATCC HB-12684) and MAb 1812-4-8 (ATCC HB-12683) specifically bind to human cytochrome P450 1A2. They inhibit 1A2 enzyme activity [U.S. Pat. 6,335,428]. None of the three MAbs cross-react with human cytochromes 1A1, 2A6, 2B6, 2C8, 2C9, 2C18, 2C19, 2D6, 2E1, 3A4, and 3A5 as measured by ELISA and immunoblotting procedures. The antibodies exhibit strong immunoblotting to 1A2 [U.S. Pat. 6,335,428]. The antibodies can be used for screening drugs for metabolism by cytochrome P450 1A6, and in methods of measuring p450 1A2 levels in individuals relative to p450 1A2 levels in a control population [U.S. Pat. 6,335,428].

Propagation: MAb 951-5-1細(xì)胞ATCC complete growth medium: RPMI 1640 medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 4.5 g/L glucose, 10 mM HEPES, 1.0 mM sodium pyruvate and supplemented with 5% Origen Hybridoma Cloning Factor and 15% fetal bovine serum

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing: Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 1 X 10(5) viable cells/ml. Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Interval: Maintain cell density between 1 X 10(5) and 1 X 10(6) viable cells/ml.

Medium Renewal: MAb 951-5-1細(xì)胞Two to three times weekly

Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 61487: Gelboin HV, et al. Agents that bind to and inhibit human cytochrome P450 1A2. US Patent 6,335,428 dated Jan 1 2002

70461: Yang TJ, et al. Inhibitory monoclonal antibodies to human cytochrome P450 1A2: analysis of phenacetin O-deethylation in human liver. Pharmacogenetics 8: 375-382, 1998. MAb 951-5-1細(xì)胞PubMed: 9825829