RIN-m5F細(xì)胞

器官來(lái)源： 胰腺

品系： NEDH

組織來(lái)源： islet of Langerhans

細(xì)胞類(lèi)型： 其他細(xì)胞類(lèi)型

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 大鼠

ATCC Number： CRL-11605?

相關(guān)**： 胰島細(xì)胞瘤

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

數(shù)量： 大量

RIN-m5F細(xì)胞Designations: RIN-m5F

Depositors: Miles, Inc.

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Organism: Rattus norvegicus deposited as Rattus sp.

Morphology: epithelial

Source: Organ: pancreas

Strain: NEDH

Tissue: islet of Langerhans

Disease: insulinoma

Cell Type: beta cell;

Cellular Products: insulin; L-dopa-decarboxylase

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. RIN-m5F細(xì)胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Gender: male

Comments: The RIN-m5F cell line was is a clone derived from the RIN-m rat islet cell line.

The cells produce and secrete insulin, and produce L-dopa-decarboxylase (a marker for cells having"amine precursor uptake and decarboxylation or APUD, activity).

Unlike the parental line they do not produce somatostatin.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Subculturing: Protocol:

Remove and discard culture medium.

RIN-m5F細(xì)胞Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37?C.

Cells will take about 3 days to attach fully. Cells attach as small islands which begin to flatten and spread into patchy epithelial morphology by day 3 or 4.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended

Medium Renewal: Two to three times weekly. Do not fluid change for 3 to 4 days after plating. Cells are loose. Just add medium.

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: RIN-m5F細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 22483: Bhathena SJ, et al. Insulin, glucagon, and somatostatin secretion by cultured rat islet cell tumor and its clones. Proc. Soc. Exp. Biol. Med. 175: 35-38, 1984. PubMed: 6141566

22592: Chick WL, et al. A transplantable insulinoma in the rat. Proc. Natl. Acad. Sci. USA 74: 628-632, 1977. PubMed: 191819

22640: Bhathena SJ, et al. Insulin, glucagon, and somatostatin receptors on cultured cells and clones from rat islet cell tumor. Diabetes 31: 521-531, 1982. PubMed: 6295859

23266: Gazdar AF, et al. Continuous, clonal, insulin- and somatostatin-secreting cell lines established from a transplantable rat islet cell tumor. Proc. Natl. Acad. Sci. USA 77: 3519-3523, 1980. PubMed: 6106192

23511: Oie HK, et al. RIN-m5F細(xì)胞Clonal analysis of insulin and somatostatin secretion and L-dopa decarboxylase expression by a rat islet cell tumor. Endocrinology 112: 1070-1075, 1983. PubMed: 6129963