DH82細胞
是否是腫瘤細胞： 0
物種來源： 狗
數(shù)量： 大量
生長狀態(tài)： 貼壁生長
運輸方式： 凍存運輸
細胞形態(tài)： 單核細胞/巨噬細胞
年限： 10 years
ATCC Number： CRL-10389?
相關(guān)**： 其他**
Designations: DH82
Depositors: The United States of America
DH82細胞Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Canis familiaris
Morphology: macrophage
Source: Disease: malignant histiocytosis
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Age: 10 years
Gender: male
Comments: DH82細胞The DH82 cell line has a macrophage-like morphology, and is able to phagocytize latex particles.
They are positive for Fc gamma receptors and negative for Fc mu and C3b receptors.
The cells do not produce IL-1.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 15%.
Temperature: 37.0°C
Subculturing: DH82細胞Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.
Add fresh culture medium, aspirate and dispense into new culture flasks.
Preservation: culture medium 95%; DMSO, 5%
Related Products: DH82細胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
References: 22019: Dawson JE, Rikihisa Y. Growing Ehrlichia species in a continuous cell line. US Patent 5,192,679 dated Mar 9 1993
22675: Wellman ML, et al. A macrophage-monocyte cell line from a dog with malignant histiocytosis. In Vitro Cell. Dev. Biol. 24: 223-229, 1988. PubMed: 3350786
