LLC-RK1細胞
生長狀態(tài)： 貼壁生長
是否是腫瘤細胞： 0
物種來源： 其他
細胞形態(tài)： 上皮樣
ATCC Number： CCL-106?
相關(guān)**： 正常
器官來源： 腎臟
數(shù)量： 大量
運輸方式： 凍存運輸
Designations: LLC-RK1
LLC-RK1細胞Depositors: RN Hull
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Oryctolagus cuniculus
Morphology: epithelial
Source: Organ: kidney
Disease: normal
Cellular Products: plasminogen activator; keratin
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. LLC-RK1細胞Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Virus Resistance: poliovirus 1
Comments: The cells are positive for keratin by immunoperoxidase staining.
The cells are negative for bovine viral diarrhea virus (BVDV). [22883]
Propagation: ATCC complete growth medium: Medium 199 with 1.12 g/L sodium bicarbonate, 90%; horse serum, 10%
Temperature: 37.0°C
Subculturing: Protocol: Please note, this cell line is extremely sensitive to trypsin.Remove the spent medium, and rinse the monolayer with fresh 0.25% trypsin, 0.53 mM EDTA solution. LLC-RK1細胞Remove the trypsin, add fresh trypsin and let the culture sit at room temperature (or at 37C) until the cells detach (about 10 minutes). Transfer the cell suspension to a centrifuge tube, and spin at approximately 125 x g for 5 to 10 minutes. Remove the supernatant, add fresh medium to the pellet, aspirate and dispense into new flasks.
Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:8 is recommended
Medium Renewal: Twice per week
References: 22354: Hull RN, et al. Development and characteristics of the rabbit kidney cell line strain, LLC-RK. Proc. Soc. Exp. Biol. Med. 118: 1054-1059, 1965. PubMed: 14277663
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
26280: Hull RN, Butorac G. LLC-RK1細胞The utility of rabbit kidney cell strain, LLC-RK to rubella virus studies. Am. J. Epidemiol. 83: 509-517, 1966. PubMed: 4956596
