HRT-18G細(xì)胞

細(xì)胞形態(tài)： 上皮樣

運(yùn)輸方式： 凍存運(yùn)輸

是否是腫瘤細(xì)胞： 0

物種來(lái)源： 人

數(shù)量： 大量

生長(zhǎng)狀態(tài)： 貼壁生長(zhǎng)

器官來(lái)源： 結(jié)腸

年限： 67 years

ATCC Number： CRL-11663?

HRT-18G細(xì)胞相關(guān)**： 其他**

Designations: HRT-18G

Depositors: Louisiana State Univ. Agricultural and Mechanical College

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens

Morphology: epithelial

Source: Organ: colon

Disease: HRT-18G細(xì)胞ileocecal colorectal adenocarcinoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimately responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: They can be used for propagation and plaque titration of bovine coronaviruses.

Age: 67 years

Gender: male

Comments: This line is a derivative of HRT-18 (ATCC CCL-244) and represents a subpopulation of cells selected by successive passage in different media.

HRT-18G cells show enhanced susceptibility to bovine coronaviruses relative to the parental HRT-18 cell line.

They can be used for propagation and plaque titration of bovine coronaviruses.

Propagation: HRT-18G細(xì)胞ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 5%.

Subculturing: Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:6 is recommended

Medium Renewal: Twice per week

Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach.

Preservation: culture medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: HRT-18G細(xì)胞Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002

Recommended serum: ATCC 30-2020

0.25% (w/v) Trypsin - 0.53mM EDTA in Hank's BSS (w/o Ca++, Mg ++): ATCC 30-2101

Cell culture tested DMSO: ATCC 4-X

Parental cell line: ATCC CCL-244